Response of Inoculated Pea Plants (pisum Sativum L.) to Root and Foliar Fertilizer Application with Reduced Molybdenum Concentration

نویسنده

  • M. Hristozkova
چکیده

The effects of foliar absorbed nutrients on root processes related to assimilation of nitrogen under presence or absence of molybdenum (Mo) were studied. Pea plants (Pisum sativum L.), var. Avola were grown until the 15th day in a glasshouse on liquid nutrient solution both without and with Mo added to the media. Plants were inoculated with bacterial suspension of Rhizobuium leguminosarum bv. Viciae strain D 293 at approximately 108 cells per cm3. Application of a liquid foliar fertilizer at a concentration of 0.3% was performed twice a week by spraying under high pressure. The effects of foliar supplied nutrients in addition to root nutrition on dry biomass accumulation, protein content, root nodulation and activities of the enzymes of primary nitrogen assimilation (nitrate reductase (NR NADH, EC 1.6.6.1), and glutamine synthetase (GS: EC 6.3.1.2) during the early stage of nodulation were studied. Foliar application of nutrients had a positive effect on the activities of NR and GS enzymes in shoots of Mo-supplied plants. It was found that foliar application of nutrients reduced the inhibitory effect of Mo shortage on root nodulation, plant dry biomass and protein content. The negative influence of Mo exclusion from the nutrient media on biomass accumulation and nodule formation was diminished through the foliar absorbed nutrients.

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تاریخ انتشار 2007